ELK Biotechnology

Mouse CAPN1(Calpain 1, Large Subunit) ELISA Kit

Product Code:
 
ELK2714
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
1 / 1
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ELK2714-48T48T£240.30£267.00
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ELK2714-96T96T£320.40£356.00
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Further Information

Alternative Names:
PIG30; CANPL1; MuCANP; MuCL; Calpain-1 Catalytic Subunit; Calcium-Dependent Protease 1; Calcium-activated neutral proteinase 1; Calpain mu-type; Micromolar-calpain
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
1.57-100 ng/mL
Sample Type:
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.62 ng/mL
Standard:
100 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CAPN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CAPN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CAPN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mouse CAPN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
O35350

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