ELK Biotechnology

Sheep NGF(Nerve Growth Factor) ELISA Kit

Product Code:
 
ELK8297
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Sheep
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
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ELK8297-48T48T£293.40£326.00
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ELK8297-96T96T£396.00£440.00
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Further Information

Alternative Names:
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
15.63-1000 pg/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
5.2 pg/mL
Standard:
1000 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep NGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sheep NGF in the samples is then determined by comparing the OD of the samples to the standard curve.

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