ELK Biotechnology

Dog IL1RA(Interleukin 1 Receptor Antagonist) ELISA Kit

Product Code:
 
ELK5899
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Canine (dog)
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
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ELK5899-48T48T£293.40£326.00
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ELK5899-96T96T£396.00£440.00
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This product comes from: China.
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Further Information

Alternative Names:
IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP; IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
31.25-2000 pg/mL
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
12.7 pg/mL
Standard:
2000 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog IL1RA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog IL1RA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog IL1RA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dog IL1RA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
Q9BEH0

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