ELK Biotechnology

Mouse NOX2(Nicotinamide Adenine Dinucleotide Phosphate Oxidase 2) ELISA Kit

Product Code:
 
ELK9852
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
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ELK9852-48T48T£240.30£267.00
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ELK9852-96T96T£320.40£356.00
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Further Information

Alternative Names:
NADPH Oxidase 2;NOX2
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Signal transduction;Enzyme & Kinase;Metabolic pathway;Tumor immunity;
Sample type:
Tissue homogenates, cell lysates and other biological fluids
Sensitivity:
0.062 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NOX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NOX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NOX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mouse NOX2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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