KRIBIOLISA™ Oxyntomodulin (Glucagon 37) ELISA

0.01 to 100 ng/ml

Colorimetric, 450nm

Immunoassay for quantitative estimation of Oxyntomodulin in human serum, plasma and tissue extracts. Oxyntomodulin (Glucagon37) is a 37-amino acid polypeptide, consisting of the glucagon molecule plus a C- terminal extension. It is produced by ileal L cells after cleavage of proglucagon by prohormone convertase 1; mice lacking prohormone convertase 1 have a reduction in oxyntomodulin, GLP-1, GLP-2, and glicentin levels. As with GLP-1, oxyntomodulin is inactivated by DPP-4. Endogenous oxyntomodulin appears to work as a satiety signal, rising after a meal About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The Oxyntomodulin ELISA is a competitive immunoassay for the determination of Oxyntomodulin. The antiserum is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific Oxyntomodulin bound.

Human serum, plasma and tissue extracts

1 ng/ml