5-ROX N-succinimidyl ester

5-Carboxy-X-rhodamine succinimidyl ester; 5-ROX SE ; 5-CXR SE

Dark violet powder.

209734-74-7

32160000

solid

GHS07

Protect from light and moisture.

H315-H319-H335

InChi=1S/C37H33N3O7/c41-29-11-12-30(42)40(29)47-37(45)22-9-10-23(26(19-22)36(43)44)31-27-17-20-5-1-13-38-15-3-7-24(32(20)38)34(27)46-35-25-8-4-16-39-14-2-6-21(33(25)39)18-28(31)35/h9-10,17-19H,1-8,11-16H2

BTTBJYLMDASSAS-UHFFFAOYSA-N

Chemical. CAS: 209734-74-7. Formula: C37H33N3O7. MW: 631.67. 5-ROX N-succinimidyl ester (5-ROX-SE) is an amine-reactive long wavelength rhodamine dye. Derivatives made with the amine-reactive carboxy-X-rhodamine (ROX), succinimidyl ester are widely used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lissamine rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red conjugates. 5-ROX-SE derivatized compounds such as proteins, nucleic acids and drugs may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. 5-ROX-SE is a labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing. 5-ROX-SE is a single isomer of ROX and increasingly preferred for labeling peptides and nucleotides because it gives better resolution in HPLC purifications that are often required in bioconjugations. Variable ratios of the 5- and 6-isomers often cause complications in the interpretation of labeling results and assay performances. Spectral data: lambdaex=578nm; lambdaem=604nm; Extinction coefficient (cm-1M-1): 82000; Quantum yield: 0.94 (aqueous buffer pH 7.0).

MFCD07437880

C37H33N3O7

631.67

Vial

P261-P264-P271-P280-P302+P352-P305+P351+P338

5-ROX N-succinimidyl ester (5-ROX-SE) is an amine-reactive long wavelength rhodamine dye. Derivatives made with the amine-reactive carboxy-X-rhodamine (ROX), succinimidyl ester are widely used for oligonucleotide labeling and automated DNA sequencing applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lissamine rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red conjugates. 5-ROX-SE derivatized compounds such as proteins, nucleic acids and drugs may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. 5-ROX-SE is a labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing. 5-ROX-SE is a single isomer of ROX and increasingly preferred for labeling peptides and nucleotides because it gives better resolution in HPLC purifications that are often required in bioconjugations. Variable ratios of the 5- and 6-isomers often cause complications in the interpretation of labeling results and assay performances. Spectral data: lambdaex=578nm; lambdaem=604nm; Extinction coefficient (cm-1M-1): 82000; Quantum yield: 0.94 (aqueous buffer pH 7.0).

TLC-Test

Warning

[O-]C(=O)c1cc(ccc1-c2c3cc4CCCN5CCCc(c45)c3[o+]c6c7CCCN8CCCc(cc26)c78)C(=O)ON9C(=O)CCC9=O

Soluble in DMF, acetonitrile or DMSO.

Synthetic

Non-hazardous

41105331

Stable for at least 2 years after receipt when stored at -20°C.