Ponasterone A

AdipoGen Life Sciences
Product Code: AG-CN2-0053
CodeSizePrice
AG-CN2-0053-M0011 mg£70.00
Quantity:
AG-CN2-0053-M0055 mg£190.00
Quantity:
AG-CN2-0053-M02525 mg£730.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Shipping:
Ambient
Storage:
-20°C

Images

1 / 1
Chemical Structure

Chemical Structure

Further Information

Alternate Names/Synonyms:
Pon A; 25-Deoxy-20-hydroxyecdysone; 25-Deoxyecdysterone; AI3-44686; 2beta,3beta,14alpha,20R,22R-Pentahydroxy-5beta-cholest-7-en-6-one
Appearance:
White to off-white powder.
CAS:
13408-56-5
EClass:
32160000
Form (Short):
liquid
InChi:
InChI=1S/C27H44O6/c1-15(2)6-7-23(31)26(5,32)22-9-11-27(33)17-12-19(28)18-13-20(29)21(30)14-24(18,3)16(17)8-10-25(22,27)4/h12,15-16,18,20-23,29-33H,6-11,13-14H2,1-5H3/t16-,18-,20+,21-,22-,23+,24+,25+,26+,27+/m0/s1
InChiKey:
PJYYBCXMCWDUAZ-JJJZTNILSA-N
Long Description:
Chemical. CAS: 13408-56-5. Formula: C27H44O6. MW: 464.6. Semi-synthetic. A member of the ecdysteroid family. Ecdysone receptor (EcR) agonist. Analog of ecdysone with similar properties to muristerone A. Functional, reliable and economical substitute for muristerone A as an inducer for the ecdysone-inducible mammalian expression system. Induces expression of beta-galactosidase to levels similar to those obtained with muristerone A induction. Potent compound for ecdysteroid activity. Insect steroid hormone involved in regulating metamorphosis.
MDL:
MFCD00272144
Molecular Formula:
C27H44O6
Molecular Weight:
464.6
Other data:
To prepare a 1mM stock solution (1.1mM) in 100% ethanol: Add 2ml of 100% ethanol to 1mg (2µmol). Gently mix to dissolve the product (5 to 10min.). Do not heat solution to dissolve the product. Store the 1mM stock solution at +4°C or at -20°C.
Package Type:
Vial
Product Description:
A member of the ecdysteroid family. Ecdysone receptor (EcR) agonist. Analog of ecdysone with similar properties to muristerone A. Functional, reliable and economical substitute for muristerone A as an inducer for the ecdysone-inducible mammalian expression system. Induces expression of beta-galactosidase to levels similar to those obtained with muristerone A induction. Potent compound for ecdysteroid activity. Insect steroid hormone involved in regulating metamorphosis.
Purity:
>95% (HPLC)
SMILES:
[H][C@@]1(CC[C@@]2(O)C3=CC(=O)[C@]4([H])C[C@@H](O)[C@@H](O)C[C@]4(C)[C@@]3([H])CC[C@]12C)[C@@](C)(O)[C@H](O)CCC(C)C
Solubility Chemicals:
Soluble in acetic acid, ethanol, methanol or DMSO. Insoluble in water.
Source / Host:
Semi-synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Natural Products/Extracts
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.

References

Extraction of ponasterone A and ecdysterone from podocarpaceae and related plants: S. Imai et al.; Steroids 10, 557 (1967) | Ecdysones and analogs: effects on development and reproduction of insects: W.E. Robbins, et al.; Science 161, 1158 (1968) | The uptake and release of ponasterone A by the Kc cell line of Drosophila melanogaster: C. Beckers, et al.; Mol. Cell. Endocrinol. 17, 51 (1980) | Past and present studies with ponasterones, the first insect molting hormones from plants: K. Nakanishi; Steroids 57, 649 (1992) | Identification of ligands and coligands for the ecdysone-regulated gene switch: E. Saez, et al.; PNAS 97, 14512 (2000) | Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and overexpression of the enzyme in Escherichia coli and mammalian cells: C. Rampazzo, et al.; J. Biol. Chem. 275, 5409 (2000) | Sustained mammary gland-directed, ponasterone A-inducible expression in transgenic mice: C. Albanese, et al.; FASEB J. 14, 877 (2000) | The ecdysone inducible gene expression system: unexpected effects of muristerone A and ponasterone A on cytokine signaling in mammalian cells: S. Constantino, et al.; Eur. Cytokine Netw. 12, 365 (2001) | Recent advances in inducible expression in transgenic mice: C. Albanese, et al.; Semin. Cell. Dev. Biol. 13, 129 (2002) | Potent and selective partial ecdysone agonist activity of chromafenozide in Sf9 cells: T. Toya, et al.; BBRC 292, 1087 (2002) | Development of a ponasterone A-inducible gene expression system for application in cultured skeletal muscle cells: Y.Y. Xiao, et al.; Int. J. Biochem. Cell. Biol. 35, 79 (2003) | Development of an in vitro Reporter Gene Assay for Screening the Ecdysone Receptor Agonists in Mysid Crustacean: M. Hirano, et al.; Terrapub 141 (2010)

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