SARS Matrix Antibody

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ProSci
Product Code: PSI-3527
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3527-0.02mg0.02mg£150.00
Quantity:
PSI-3527-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Images

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<strong>Figure 1 ELISA Test </strong><br> Antibodies: SARS-CoV Matrix Antibody, 3527 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.
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<strong>Figure 1 ELISA Test </strong><br> Antibodies: SARS-CoV Matrix Antibody, 3527 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.

Documents

Further Information

Additional Names:
SARS-CoV Matrix Antibody: Membrane protein, E1 glycoprotein, M protein
Application Note:
SARS-CoV Matrix antibody can be used for the detection of SARS-CoV Matrix protein in ELISA. It will detect 5 ng of free peptide at 1 μg/mL.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. The membrane (M) protein or matrix protein is the most abundant structural protein and defines the shape of the viral envelope (3). It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein. It involves in organization of the nucleoprotein inside, which includes many copies of the N (nucleocapsid) protein bound to the genomic RNA. The M protein holds dominant cellular immunogenicity and has been determined as a protective antigen in humoral responses, which suggests it would serve as a potential target in vaccine design (4).
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
  • Neuman, et al. J Struct Biol. 2011;174(1):11?22.
  • Liu et al. J Infect Dis. 2010;202(8):1171-80.
Buffer:
SARS-CoV Matrix Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV-2 Matrix protein: (identity 77%, homology 93%)
Immunogen:
Anti-SARS-CoV Matrix antibody (3527) was raised against a peptide corresponding to 13 amino acids near the amino-terminus of SARS-CoV Matrix protein.

The immunogen is located within the first 50 amino acids of SARS-CoV Matrix.
ISOFORMS:
SARS-CoV Matrix has one isoform (222aa).
NCBI Gene ID #:
1489672
NCBI Official Name:
Membrane protein
NCBI Official Symbol:
M
NCBI Organism:
SARS coronavirus
Physical State:
Liquid
Protein Accession #:
P59596
Protein GI Number:
30173398
Purification:
SARS-CoV Matrix Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P59596
User NOte:
Optimal dilutions for each application to be determined by the researcher.

References

  1. Jaume, et al. Anti-severe acute respiratory syndrome coronavirus spike antibodies trigger infection of human immune cells via a pH- and cysteine protease-independent Fc?R pathway. J Virol. 2011 Oct;85(20):10582-97. doi: 10.1128/JVI.00671-11. Epub 2011 Jul 20.PMID: 21775467
  2. Quijano-Rubio, et al. De novo design of modular and tunable allosteric biosensors. bioRxiv. 2020 Jul 20;2020.07.18.206946. doi: 10.1101/2020.07.18.206946.PMID: 32743576

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