ProSci

ZBP1 Antibody

Product Code:
 
PSI-4401
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
ZBP1 antibody can be stored at 4˚C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
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<strong>Figure 1 WB Validation in Human Tonsil</strong><br> Loading: 10 μg of lysate. Antibodies: ZBP1 4401, 1 μg/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 2 Western Blot Validation in Human Brain</strong><br> Loading: 10 μg of lysates per lane. Antibodies: ZBP1 4401, 1 μg/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
3 / 9
<strong>Figure 3 Western Blot Validation in Mouse Brain </strong><br> Loading: 10 μg of lysates per lane. Antibodies: ZBP1 4401, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 4 WB Validation in Rat Brain</strong><br> Loading: 10 μg of lysate. Antibodies: ZBP1 4401, 1 μg/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution
5 / 9
<strong>Figure 5 WB Validation in A549 Cells</strong><br> Loading: 10 μg of lysate. Antibodies: ZBP1 4401, 1 μg/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution
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<strong>Figure 6 Immunohistochemistry Validation of ZBP1 in Human Tonsil</strong><br> Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZBP1 antibody (4401) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 7 Immunohistochemistry Validation of ZBP-1 in Mouse Brain</strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ZBP1 antibody (4401) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
8 / 9
<strong>Figure 8 Immunofluorescence Validation of ZBP1 in Human K562 Cells </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 labeling ZBP1 with 4401 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 9 Immunofluorescence Validation of ZBP1 in Rat Brain </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling ZBP1 with 4401 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 1 WB Validation in Human Tonsil</strong><br> Loading: 10 μg of lysate. Antibodies: ZBP1 4401, 1 μg/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Western Blot Validation in Human Brain</strong><br> Loading: 10 μg of lysates per lane. Antibodies: ZBP1 4401, 1 μg/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse Brain </strong><br> Loading: 10 μg of lysates per lane. Antibodies: ZBP1 4401, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4 WB Validation in Rat Brain</strong><br> Loading: 10 μg of lysate. Antibodies: ZBP1 4401, 1 μg/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution
<strong>Figure 5 WB Validation in A549 Cells</strong><br> Loading: 10 μg of lysate. Antibodies: ZBP1 4401, 1 μg/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution
<strong>Figure 6 Immunohistochemistry Validation of ZBP1 in Human Tonsil</strong><br> Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZBP1 antibody (4401) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of ZBP-1 in Mouse Brain</strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ZBP1 antibody (4401) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunofluorescence Validation of ZBP1 in Human K562 Cells </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 labeling ZBP1 with 4401 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 9 Immunofluorescence Validation of ZBP1 in Rat Brain </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling ZBP1 with 4401 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

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PSI-4401-0.02mg0.02mg£150.00
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PSI-4401-0.1mg0.1mg£453.00
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Further Information

Additional Names:
ZBP1 Antibody: DAI, DLM1, DLM-1, C20orf183, Z-DNA-binding protein 1, Tumor stroma and activated macrophage protein DLM-1
Application Note:
WB: 1-2 μg/mL; IHC-P/IF: 5-20 μg/mL; IHC-P: 1-5 μg/mL; ICC/IF: 10-20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human and rat samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.
Background:
Z-DNA Binding Protein 1 (ZBP1), or DNA-dependent activator of IFN-regulatory factors (DAI), is a pivotal protein in innate immunity and stress response. With two Z-DNA binding domains, ZBP1 senses cytosolic nucleic acids, triggering type I interferon responses. Beyond immunity, ZBP1 participates in necroptosis, a regulated cell death pathway. Dysregulation of ZBP1 is linked to autoimmune disorders and viral infections, making it a key player in disease pathogenesis.
Background References:
  • Takaoka A, et al. (2007). Nature, 448(7152), 501-505. DOI: 10.1038/nature06013
  • Zhang Z, et al. (2011). Nature Immunology, 12(10), 959-965. DOI: 10.1038/ni.2091
  • Kaiser WJ, et al. (2013). Nature, 471(7338), 368-372. DOI: 10.1038/nature09857
  • Lin J, et al. (2016). Nature, 540(7631), 124-128. DOI: 10.1038/nature20558
Buffer:
ZBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ZBP1 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human ZBP1.

The immunogen is located within the last 50 amino acids of ZBP1.
Isoforms:
Human ZBP1 has 7 isoforms, including isoform 1 (429aa, 46kD), isoform 2 (446aa, 49kD), isoform 3 (380aa, 41kD), isoform 4 (406aa, 44kD), isoform 5 (149aa, 16kD), isoform 6 (248aa, 27kD), and isoform 7 (354aa, 38kD). Mouse ZBP1 has 2 isoform (411aa, 44kD) and isoform 2 (187aa, 21kD). Rat ZBP1 has 1 isoform (409aa, 44kD). 4401 can detect ZBP1 in human, mouse, and rat.
NCBI Gene ID #:
81030
NCBI Official Name:
Z-DNA binding protein 1
NCBI Official Symbol:
ZBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
Predicted: 46 kD
Observed: 46 kD
Protein Accession #:
EAW75510
Protein GI Number:
119595916
Purification:
ZBP1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
SPECIFICITY:
Multiple isoforms of ZBP1 are known to exist.
Swissprot #:
Q9H171
User NOte:
Optimal dilutions for each application to be determined by the researcher.