anti-Apaf-1 (human) mAb (2E12)

AdipoGen Life Sciences
Product Code: AG-20T-0132
Product Group: Primary Antibodies
CodeSizePrice
AG-20T-0132-C100100 ug£270.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
-20°C
Storage:
-20°C

Images

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Western blot analysis using anti-Apaf-1 (human), MAb (2E12) (AG-20T-0132) on several human cell lines and one monkey cell line (COS).

Western blot analysis using anti-Apaf-1 (human), MAb (2E12) (AG-20T-0132) on several human cell lines and one monkey cell line (COS).

Further Information

Alternate Names/Synonyms:
Apoptosis Protease-activating Factor 1
Concentration:
1mg/ml
EClass:
32160000
Endotoxin:
<0.1EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.
Long Description:
Monoclonal Antibody. Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1. Isotype: Rat IgG2akappa. Clone: 2.00E+12. Applications: ELISA, ICC, IHC, IP, WB. Liquid. In PBS containing 0.02% sodium azide. Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
NCBI, Uniprot Number:
O14727
Package Type:
Plastic Vial
Product Description:
Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
Purity:
>95% (SDS-PAGE)
Specificity:
Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.

References

Bcl-2 family members do not inhibit apoptosis by binding the caspase activator Apaf-1: K. Moriishi, et al.; PNAS 96, 9683 (1999) | Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol. 49, 623 (2000) | Apaf-1 is a transcriptional target for E2F and p53: M.C. Moroni, et al.; Nat. Cell Biol. 3, 552 (2001) | Is Apaf-1 expression frequently abrogated in melanoma?: J.D. Allen, et al.; Cell Death Differ. 12, 680 (2005) | Differential Apaf-1 levels allow cytochrome c to induce apoptosis in brain tumors but not in normal neural tissues: C.E. Johnson, et al.; PNAS 104, 20820 (2007)