CD80 Recombinant Protein

Product Code: PSI-96-044

Supplier: ProSci

Code	Size	Price

PSI-96-044-0.1mg

0.1mg

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO

Target Species: Human

Application: Western Blot (WB)

Storage:

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Documents

Datasheet

Further Information

Additional Names:

CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7

Application Note:

This recombinant protein can be used for WB. For research use only.

Background:

B7-1 and B7-2, together with their receptors CD28 and CTLA?4, constitute one of the dominant co-stimulatory pathways that regulate T? and B?cell responses. Although both CTLA?4 and CD28 can bind to the same ligands, CTLA?4 binds to B7?1 and B7?2 with a 20 ? 100 fold higher affinity than CD28 and is involved in the down?regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Background References:

Peach RJ., et al., 1995, J. Biol. Chem. (UNITED STATES) 270 (36): 21181?7.
Stamper CC., et al., 2001, Nature (England) 410 (6828): 608?11.
Bhatia S., et al., 2005, Proc. Natl. Acad. Sci. 102: 15569-15574.
Khan N., et al., 2007, Int. Immunol. 19:477-486.

Biological Activity:

Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, His Tag at 20ug/mL (100 ul/well),can bind Human CD28, Fc Tag with a linear of 0.2-20 ug/mL. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells. The?ED50 for?this?effect?is?typically?0.01 - 0.1?ug/mL?in?the?presence?of?PHA.

Buffer:

PBS, pH7.4

Fusion Tag:

His Tag

NCBI Gene ID #:

941

NCBI Official Name:

CD80 molecule

NCBI Official Symbol:

CD80

NCBI Organism:

Homo sapiens

Physical State:

Lyophilized

Predicted Molecular Weight:

25.8 kDa

Protein Accession #:

P33681-1

Purity:

>90% as determined by SDS-PAGE.

Less than 1.0 EU per ug by the LAL method.

Recombinant Protein Sequence:

Thr32-Gly402

Source:

HEK293 cells

Swissprot #:

P33681