ProSci

BCLAF1 Antibody

Product Code:
 
PSI-8373
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
BCLAF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
1 / 2
Western blot analysis of BCLAF1 in human brain tissue lysate with BCLAF1 antibody at 1 μg/ml.
2 / 2
Immunohistochemistry of BCLAF1 in mouse brain tissue with BCLAF1 antibody at 5 μg/mL.
❮❯

Western blot analysis of BCLAF1 in human brain tissue lysate with BCLAF1 antibody at 1 μg/ml.
Immunohistochemistry of BCLAF1 in mouse brain tissue with BCLAF1 antibody at 5 μg/mL.

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CodeSizePrice
PSI-8373-0.02mg0.02mg£150.00
Quantity:
PSI-8373-0.1mg0.1mg£453.00
Quantity:
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Further Information

Additional Names:
BCL2-associated transcription factor 1
Application Note:
BCLAF1 antibody can be used for detection of BCLAF1 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
The BCL2-associated transcription factor 1 (BCLAF1) is a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins (1). BCLAF1 localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. (2). More recent studies have suggested BCLAF1 plays roles in processes not associated with actions of the BCL2 family members, including lung development, T cell activation, and post-transcriptional processes that affect mRNA metabolism (3).
Background References:
  • Kasof GM, Goyal L, and White E. Btf, a novel death-promoting transcriptional repressor that interacts with Bcl-2-related proteins. Mol. Cell Biol. 1999; 19:4390-404.
  • Haraguchi T, holaska JM, Yamana M, et al. Emerin binding to Btf, a death-promoting transcriptional repressor, is disrupted by a missense mutation that causes Emery-Dreifuss muscular dystrophy. Eur. J. Biochem. 2004; 271:1035-45.
  • Sarras H, Azami SA, and McPherson JP. In search of a function for BCLAF1. The Scientific World Journal 2010; 10:1450-61.
Buffer:
BCLAF1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BCLAF1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human BCLAF1.

The immunogen is located within amino acids 820 - 870 of BCLAF1.
NCBI Gene ID #:
9774
NCBI Official Name:
BCL2-associated transcription factor 1
NCBI Official Symbol:
BCLAF1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 101 kDa

Observed: 105 kDa
Protein Accession #:
NP_055554
Protein GI Number:
7661958
Purification:
BCLAF1 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell,Apoptosis
SPECIFICITY:
BCLAF1 antibody is human, mouse and rat reactive. At least four isoforms of BCLAF1 are known to exist; this antibody will detect all but isoform 2.
Swissprot #:
Q9NYF8
User NOte:
Optimal dilutions for each application to be determined by the researcher.