Human 5-MTHF (5-Methyltetrahydrofolate) ELISA Kit

L-5-MTHF; 5-MTHF;

3.5h

Sandwich

0.16-10 ng/mL

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

0.059 ng/mL

10 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5-MTHF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5-MTHF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5-MTHF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human 5-MTHF in the samples is then determined by comparing the OD of the samples to the standard curve.

P49914