EcoPlexTM Human Inflammation 6-Plex Panel Kit

3 h

Cross-reactivity of analytes in the Panel: Negligible

Intra-assay CV<10% Inter-assay CV<20%

70%-130%

TNF-α<1pg/mL IL-1β<5pg/mL IL-8<1pg/mL IL-12p70<3pg/mL IL-6<5pg/mL IL-10<2pg/mL

Human

EcoPlexTM CBA kits utilize antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.

Serum,plasma,bodily fluid,cell culture supernatant

TNF-α:P01375 IL-1β:P01584 IL-8:P10145 IL-12p70:P29459P29460 IL-6:P05231 IL-10:P22301

This kit is for detection of IL-1β; IL-6; IL-8; IL-10; IL-12p70 and TNF-α in human serum; plasma; biological fluid and cell culture supernatant.