Chemodex

Sulforhodamine B sodium salt

Product Code:
 
CDX-S0005
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
Short term: +20°C. Long term: +20°C
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Chemical Structure
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Chemical Structure

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CDX-S0005-G0055 g£116.00
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CDX-S0005-G02525 g£325.00
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This product comes from: Switzerland.
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  • Further Information
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Further Information

Appearance:
Powder.
CAS:
3520-42-1
Description:
Sulforhodamine B (SRB) is a water-soluble, anionic red fluorescent aminoxanthene dye. It is often used as a membrane-impermeable polar tracer or for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay is extensively used for in vitro cytotoxicity screening and in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions. Thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. Spectral Data: lambdaex 560nm, lambdaem 580nm.
EClass:
32160000
Form:
solid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
InChiKey:
SXQCTESRRZBPHJ-UHFFFAOYSA-M
MDL:
MFCD00010180
Molecular Formula:
C27H29N2NaO7S2
Molecular Weight:
580.65
Package Type:
Vial
Purity:
>95% (NMR)
SMILES:
CCN(CC)C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=C(S([O-])(=O)=O)C=C(S([O-])(=O)=O)C=C4)=C2C=C1.[Na+]
Solubility Chemicals:
Soluble in DMSO, ethanol, DMF or water (all 10mg/ml).
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at RT.

Documents

References

(1) P. Skehan, et al.; J. Natl. Cancer Inst. 82, 1107 (1990) | (2) S.P. Fricker; Toxicol. In Vitro 8, 821 (1994) | (3) W. Voigt; Methods Mol. Med. 110, 39 (2005) | (4) V. Vichai & K. Kirtikara; Nat. Protoc. 1, 1112 (2006) | (5) E.A. Orellana & A.L. Kasinski; Bio. Protoc. 6, e1984 (2016)