InVivoKines™ IL-18BP (mouse) (monomeric):Fc (LALA-PG)-KIH (human) (rec.)

After reconstitution: 1mg/ml

IL-18, initially named interferon-gamma inducing factor (IGIF) is an IL-1 family member and a cytokine that links innate and adaptive immunity. Depending on the microenvironment and timing, IL-18 can either drive harmful inflammation or restore immune homeostasis. IL-18 is regulated i) by the inflammasome that converts the inactive pro-IL-18 to its active form IL-18; ii) by the cytokine it induces, the master cytokine IFN-gamma that triggers the expression of the inhibitor of IL-18, called IL-18 binding protein (IL-18BP). IL-18 binding protein (IL-18BP) is one of only four natural cytokine antagonists. IL-18BP has an exceptionally high affinity for IL-18 and its slow dissociation rate makes it an effective regulator of IL-18, essential for maintaining immune balance and influencing disease outcomes. The protein IL-18BP (mouse) (monomeric):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-18BP (mouse):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 70kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into CHO cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996) required for dimerization of the Fc moieties and for secretion of the final protein IL-18BP (mouse) (monomeric):Fc-KIH (human) (rec.). The LALA-PG mutations inhibit binding to FcgammaRs and C1q, while FcRn binding and Fc stability remain unaffected. InVivoKines? are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical, and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

32160000

<0.01EU/ug protein (LAL test).

solid

Lyophilized. Contains PBS.

After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.

Plastic Vial

>95% (SDS-PAGE)

Mouse IL-18BP (aa 29-193) is fused at the C-terminus to the Fc portion of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).

CHO cells. Produced using animal component-free medium.

Mouse

Binds to mouse IL-18. Fc (LALA-PG)-KIH (human) does not bind to FcRs.

Fc

Non-hazardous

Q9Z0M9

41116127

Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.