NSJ Bioreagents

Anti-EpCAM Antibody / Extracellular domain

Product Code:
 
NSJ-V3525IHC
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
EGP40/1372
Regulatory Status:
 
RUO
Target Species:
  • Canine (dog)
  • Feline (cat)
  • Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the anti-EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 6
IHC testing of FFPE human hepatocellular carcinoma and anti-EpCAM antibody (clone EGP40/1372). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
2 / 6
IHC testing of FFPE human colorectal carcinoma and anti-EpCAM antibody (clone EGP40/1372). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
3 / 6
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using the anti-EpCAM antibody (clone EGP40/1372). These results demonstrate the foremost specificity of the EGP40/1372 mAb.<P><P>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 6
SDS-PAGE analysis of purified, BSA-free anti-EpCAM antibody (clone EGP40/1372) as confirmation of integrity and purity.
5 / 6
IHC testing of FFPE dog bladder and EpCAM antibody (clone EGP40/1372). HIER: steam sections in pH9 EDTA for 10-20 min.
6 / 6
IHC testing of FFPE cat small intestine and EpCAM antibody (clone EGP40/1372). HIER: steam sections in pH9 EDTA for 10-20 min.
❮❯

IHC testing of FFPE human hepatocellular carcinoma and anti-EpCAM antibody (clone EGP40/1372). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE human colorectal carcinoma and anti-EpCAM antibody (clone EGP40/1372). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using the anti-EpCAM antibody (clone EGP40/1372). These results demonstrate the foremost specificity of the EGP40/1372 mAb.<P><P>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free anti-EpCAM antibody (clone EGP40/1372) as confirmation of integrity and purity.
IHC testing of FFPE dog bladder and EpCAM antibody (clone EGP40/1372). HIER: steam sections in pH9 EDTA for 10-20 min.
IHC testing of FFPE cat small intestine and EpCAM antibody (clone EGP40/1372). HIER: steam sections in pH9 EDTA for 10-20 min.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V3525IHC-7ML7 ml£537.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Titering of the anti-EpCAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Format:
Purified
Formulation:
Prediluted in 1X PBS, 0.1 mg/ml BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Immunogen:
A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the anti-EpCAM antibody.
Limitation:
This anti-EpCAM antibody is available for research use only.
Localisation:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P16422

Documents