NSJ Bioreagents

Dnmt3a Antibody

Product Code:
 
NSJ-V3827SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-DNMT3A-1E2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Dnmt3a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
IHC testing of FFPE human placenta with Dnmt3a antibody. Required HIER: boil sections in 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.
2 / 4
IHC testing of FFPE human placenta with Dnmt3a antibody. Required HIER: boil sections in 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Dnmt3a antibody. These results demonstrate the foremost specificity of the PCRP-DNMT3A-1E2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free Dnmt3a antibody as confirmation of integrity and purity.
❮❯

IHC testing of FFPE human placenta with Dnmt3a antibody. Required HIER: boil sections in 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.
IHC testing of FFPE human placenta with Dnmt3a antibody. Required HIER: boil sections in 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Dnmt3a antibody. These results demonstrate the foremost specificity of the PCRP-DNMT3A-1E2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Dnmt3a antibody as confirmation of integrity and purity.

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NSJ-V3827SAF-100UG100 ug£537.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western Blot: 1-2ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Dnmt3a antibody to be titered up or down for optimal performance.
Description:
DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full length human protein was used as the immunogen for this Dnmt3a antibody.
Limitation:
This Dnmt3a antibody is available for research use only.
Localisation:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
Q16655

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