NSJ Bioreagents

Fibronectin Antibody

Product Code:
 
NSJ-V7408
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
616
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Fibronectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC staining of FFPE human prostate carcinoma with Fibronectin antibody (clone 616). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling prior to testing.
2 / 2
SDS-PAGE analysis of purified, BSA-free Fibronectin antibody (clone 616) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human prostate carcinoma with Fibronectin antibody (clone 616). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling prior to testing.
SDS-PAGE analysis of purified, BSA-free Fibronectin antibody (clone 616) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V7408-20UG20 ug£276.00
Quantity:
NSJ-V7408-100UG100 ug£537.00
Quantity:
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the Fibronectin antibody should be determined by the researcher.

1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This mAb does not cross-react with MMP-2, MMP-9, or TIMP-2.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
High molecular weight proteins secreted by cultivated human fibroblasts were used as the immunogen for the Fibronectin antibody. The epitope of this mAb is located in the 29kDa N-terminal fibrin- and heparin-binding domain of fibronectin.
Limitation:
This Fibronectin antibody is available for research use only.
Localisation:
Connective tissue matrix
Purity:
Protein G affinity chromatography
Uniprot #:
P02751

Documents