NSJ Bioreagents

Anti-CK19 Antibody

Product Code:
 
NSJ-V9126SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, lambda
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM266
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the anti-CK19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC analysis of formalin-fixed, paraffin-embedded human endometrial carcinoma stained with anti-CK19 antibody (clone SPM266).
2 / 3
Western blot testing of human HepG2 lysate with anti-CK19 antibody (clone SPM266).
3 / 3
FACS staining of methanol-fixed human MCF7 cells with anti-CK19 antibody (red, clone SPM266) and isotype control (blue).
❮❯

IHC analysis of formalin-fixed, paraffin-embedded human endometrial carcinoma stained with anti-CK19 antibody (clone SPM266).
Western blot testing of human HepG2 lysate with anti-CK19 antibody (clone SPM266).
FACS staining of methanol-fixed human MCF7 cells with anti-CK19 antibody (red, clone SPM266) and isotype control (blue).

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V9126SAF-100UG100 ug£537.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details:
Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The optimal dilution of the anti-CK19 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
The human breast cancer cell line MCF-7 was used as the immunogen for this anti-CK19 antibody. Its epitope maps between amino acids 312-335.
Limitation:
This anti-CK19 antibody is available for research use only.
Localisation:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P08727

Documents