ProSci

DOK1 Antibody

Product Code:
 
PSI-1107
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 μg/mL.
2 / 3
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 μg/mL.
3 / 3
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 μg/ml.
❮❯

Western blot analysis of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 μg/mL.
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 μg/mL.
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 μg/ml.

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CodeSizePrice
PSI-1107-0.02mg0.02mg£150.00
Quantity:
PSI-1107-0.1mg0.1mg£453.00
Quantity:
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Further Information

Additional Names:
DOK1 Antibody: Docking protein 1, Downstream of tyrosine kinase 1, P62(dok), pp62
Application Note:
DOK1 antibody can be used for detection of DOK1 expression by Western blot at 1 μg/mL. A 62 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
DOK1 Antibody: Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.
Background References:
  • Carpino N, Wisniewski D, Strife A, Marshak D, Kobayashi R, Stillman B, Clarkson B p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Cell 1997;88:197-204.
  • Yamanashi Y, Baltimore D Identification of the Abl- and rasGAP-associated 62 KDa protein as a docking protein, Dok. Cell 1997;88:205-211.
  • Holland SJ, Gale NW, Gish GD, Roth RA, Songyang Z, Cantley LC, Henkemeyer M, Yancopoulos GD, Pawson T. Juxtamembrane tyrosine residues couple the Eph family receptor EphB2/Nuk to specific SH2 domain proteins in neuronal cells. EMBO J 1997;16:3877-3888.
  • Di Cristofano A, Carpino N, Dunant N, Friedland G, Kobayashi R, Strife A, Wisniewski D, Clarkson B, Pandolfi PP, Resh MD. Molecular cloning and characterization of p56(dok-2) defines a new family of RasGAP-binding proteins. J Biol Chem 1998;273:4827-4830.
Buffer:
DOK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DOK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DOK1.

The immunogen is located within amino acids 410 - 460 of DOK1.
NCBI Gene ID #:
1796
NCBI Official Name:
docking protein 1
NCBI Official Symbol:
DOK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
Q99704.1
Protein GI Number:
17366642
Purification:
DOK1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Cancer
Swissprot #:
Q99704
User NOte:
Optimal dilutions for each application to be determined by the researcher.