ProSci

LAMP-1 Antibody

Product Code:
 
PSI-3629
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 5
Western blot analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 μg/mL.
2 / 5
Immunofluorescence of LAMP-1 in human colon tissue with LAMP-1 antibody at 20 μg/mL.
3 / 5
Immunofluorescence of LAMP-1 in Human Colon cells with LAMP-1 antibody at 20 μg/mL.
4 / 5
Immunohistochemistry of LAMP-1 in rat colon tissue with LAMP-1 antibody at 2.5 μg/ml.
5 / 5
Immunofluorescence of LAMP-1 in rat colon tissue with LAMP-1 antibody at 20 μg/ml.
❮❯

Western blot analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of LAMP-1 in human colon tissue with LAMP-1 antibody at 20 μg/mL.
Immunofluorescence of LAMP-1 in Human Colon cells with LAMP-1 antibody at 20 μg/mL.
Immunohistochemistry of LAMP-1 in rat colon tissue with LAMP-1 antibody at 2.5 μg/ml.
Immunofluorescence of LAMP-1 in rat colon tissue with LAMP-1 antibody at 20 μg/ml.

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CodeSizePrice
PSI-3629-0.02mg0.02mg£150.00
Quantity:
PSI-3629-0.1mg0.1mg£453.00
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Further Information

Additional Names:
LAMP-1 Antibody: LAMPA, CD107a, LGP120, Lysosome-associated membrane glycoprotein 1, CD107 antigen-like family member A, LAMP-1
Application Note:
LAMP-1 antibody can be used for the detection of LAMP-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
Background:
LAMP-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome. LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression. However, the loss of both proteins resulted in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.
Background References:
  • Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
  • Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.
  • Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
  • Chen JW, Murphy TL, Willingham MC, et al. Identification of two lysosomal membrane glycoproteins. J. Cell Biol. 1985; 101:85-95.
Buffer:
LAMP-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LAMP-1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human LAMP-1.

The immunogen is located within amino acids 230 - 280 of LAMP-1.
NCBI Gene ID #:
3916
NCBI Official Name:
lysosomal-associated membrane protein 1
NCBI Official Symbol:
LAMP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46 kDa

Observed: 95 kDa
Protein Accession #:
NP_005552
Protein GI Number:
112380628
Purification:
LAMP-1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Autophagy
SPECIFICITY:
LAMP-1 antibody is predicted to not cross-react with LAMP-2. Due to extensive post-translational modification, LAMP-1 often migrates at higher than predicted molecular weight in SDS-PAGE.
Swissprot #:
P11279
User NOte:
Optimal dilutions for each application to be determined by the researcher.