ProSci

Wnt10a Antibody

Product Code:
 
PSI-4639
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Wnt10a antibody can be stored at 4˚C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of Wnt10a in RAW264.7 cell lysate with Wnt10a antibody at (A) 1 and (B) 2 μg/mL.
2 / 4
Immunohistochemistry of Wnt10a in human skeletal muscle tissue with Wnt10a antibody at 10 μg/mL.
3 / 4
Immunofluorescence of Wnt10a in Human Skeletal Muscle tissue with Wnt10a antibody at 20 μg/mL.
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Immunohistochemistry of Wnt10a in mouse skeletal muscle tissue with Wnt10a antibody at 5 μg/ml.
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Western blot analysis of Wnt10a in RAW264.7 cell lysate with Wnt10a antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of Wnt10a in human skeletal muscle tissue with Wnt10a antibody at 10 μg/mL.
Immunofluorescence of Wnt10a in Human Skeletal Muscle tissue with Wnt10a antibody at 20 μg/mL.
Immunohistochemistry of Wnt10a in mouse skeletal muscle tissue with Wnt10a antibody at 5 μg/ml.

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CodeSizePrice
PSI-4639-0.02mg0.02mg£150.00
Quantity:
PSI-4639-0.1mg0.1mg£453.00
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Further Information

Additional Names:
Wnt10a Antibody: OODD, SSPS, STHAG4, Protein Wnt-10a
Application Note:
Wnt10a antibody can be used for detection of Wnt10a by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Wnt10a Antibody: Wnt10a is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Elevated levels of Wnt10a have been detected in several human tumors and cancer cell lines including colorectal cancer and chronic lymphocytic leukemia and it has been suggested that uncontrolled Wnt signaling may contribute to the defect in apoptosis that characterizes these malignancies. Recently, a mutation in Wnt10a was found to be associated with odonto-onycho-dermal dysplasia, an autosomal recessive ectodermal dysplasia.
Background References:
  • Kelly GM, Lai CJ, and Moon RT. Expression of wnt10a in the central nervous system of developing zebrafish. Dev. Biol. 1993; 158:113-21.
  • Nusse R and Varmus RE. Wnt genes. Cell 1992; 69:1073-87.
  • Kirikoshi H, Sekihara H, and Katoh M. WNT10A and WNT6, clustered in human chromosome 2q35 region head-to-tail manner, are strongly coexpressed in SW480 cells. Biochem. Biophys. Res. Commun. 2001; 283:798-805.
  • Kirikoshi H, Inoue S, Sekihara H, et al. Expression of WNT10A in human cancer. Int. J. Oncol. 2001; 19:997-1001.
Buffer:
Wnt10a Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Wnt10a antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Wnt10a.

The immunogen is located within amino acids 300 - 350 of Wnt10a.
NCBI Gene ID #:
80326
NCBI Official Name:
wingless-type MMTV integration site family, member 10A
NCBI Official Symbol:
WNT10A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46 kDa

Observed: 46 kDa
Protein Accession #:
Q9GZT5
Protein GI Number:
14424011
Purification:
Wnt10a Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Cancer
SPECIFICITY:
This Wnt10a antibody is predicted to not cross-react with Wnt10b.
Swissprot #:
Q9GZT5
User NOte:
Optimal dilutions for each application to be determined by the researcher.