ProSci

ZHANGFEI Antibody

Product Code:
 
PSI-6191
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 2
Western blot analysis of ZHANGFEI in K562 cell lysate with ZHANGFEI antibody at 1 μg/mL.
2 / 2
Immunocytochemistry of ZHANGFEI in K562 cells with ZHANGFEI antibody at 10 μg/mL.
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Western blot analysis of ZHANGFEI in K562 cell lysate with ZHANGFEI antibody at 1 μg/mL.
Immunocytochemistry of ZHANGFEI in K562 cells with ZHANGFEI antibody at 10 μg/mL.

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CodeSizePrice
PSI-6191-0.02mg0.02mg£150.00
Quantity:
PSI-6191-0.1mg0.1mg£453.00
Quantity:
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Further Information

Additional Names:
ZHANGFEI Antibody: ZF, SMILE, ZF, Host cell factor-binding transcription factor Zhangfei, HCF-binding transcription factor Zhangfei
Application Note:
ZHANGFEI antibody can be used for detection of ZHANGFEI by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
ZHANGFEI Antibody: ZHANGFEI, also known as ZF, is a basic region-leucine zipper transcriptional factor that has been implicated in herpes virus infection cycle and related cellular processes. ZHANGFEI protein has a negatively charged active N-terminus, a leucine zipper of six heptad leucine repeats separated by a conserved six amino acid spacer, a basic domain and a bZIP region. ZHANGFEI strongly activates transcription and suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1 dependent manner. It also suppresses the HCFC1 dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell.
Background References:
  • Hogan MR, Cockram GP and Lu R. Cooperative interaction of Zhangfei and ATF4 in transactivation of the cyclic AMP response element. FEBS Lett. 2006; 580:58-62.
  • Cockram GP, Hogan MR, Burnett HF, et al. Identification and characterization of the DNA-binding properties of a Zhangfei homologue in Japanese pufferfish, Takifugu rubripes. Biochem. Biophys. Res. Commun. 2005; 339:1238-45.
  • Lu R and Misra V. Zhangfei: a second cellular protein interacts with herpes simplex virus accessory factor HCF in a manner similar to Luman and VP16. Nucleic Acids Res. 2000; 28:2446-54.
  • Misra V, Rapin N, Akhova O, et al. 2005. Zhangfei is a potent and specific inhibitor of the host cell factor-binding transcription factor Luman. J. Biol. Chem. 2005; 280:15257-66.
Buffer:
ZHANGFEI Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ZHANGFEI antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human ZHANGFEI.

The immunogen is located within amino acids 180 - 230 of ZHANGFEI.
NCBI Gene ID #:
58487
NCBI Official Name:
CREB/ATF bZIP transcription factor
NCBI Official Symbol:
CREBZF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001034707
Protein GI Number:
88900495
Purification:
ZHANGFEI Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
Swissprot #:
Q9NS37
User NOte:
Optimal dilutions for each application to be determined by the researcher.