ProSci

SIK3 Antibody

Product Code:
 
PSI-6613
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Chicken
Antibody Isotype:
 
IgY
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
1 / 2
Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 2
Immunofluorescence of SIK3 in rat brain cells with SIK3 antibody at 20 μg/mL.
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Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of SIK3 in rat brain cells with SIK3 antibody at 20 μg/mL.

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CodeSizePrice
PSI-6613-0.02mg0.02mg£150.00
Quantity:
PSI-6613-0.1mg0.1mg£453.00
Quantity:
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Further Information

Additional Names:
SIK3 Antibody: L19, QSK, SIK-3, KIAA0999, L19, Salt-inducible kinase 3
Application Note:
SIK3 antibody can be used for detection of SIK3 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
SIK3 Antibody: Salt-inducible kinase family (SIKs) proteins are thought to have a role in steroidogenesis, adipogenesis or regulation of tumor malignancy. Three members (SIK1, SIK2 and SIK3) in the SIK family have been identified thus far. Their kinase domain sequences are closely homologous to those of AMP-activated protein kinase (AMPK). SIK3 can be phosphorylated by a tumor-suppressor kinase LKB1. It is highly and preferentially expressed in ovarian tumors but not in adenomyosis and leiomyoma and may be a potential diagnostic marker for ovarian cancers.
Background References:
  • Katoh Y, Takemori H, Horike N, et al. Salt-inducible kinase (SIK) isoforms: their involvement in steroidogenesis and adipogenesis. Mol. Cell. Endocrinol. 2004; 217:109-12.
  • Al-Hakim AK, Göransson O, Deak M, et al. 14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK. J. Cell Sci. 2005; 118:5661-73.
  • Charoenfuprasert S, Yang YY, Lee YC, et al. Identification of salt-inducible kinase 3 as a novel tumor antigen associated with tumorigenesis of ovarian cancer. Oncogene 2011; 30:3570-84.
Buffer:
SIK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SIK3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SIK3.

The immunogen is located within amino acids 1190 - 1240 of SIK3.
NCBI Gene ID #:
23387
NCBI Official Name:
SIK family kinase 3
NCBI Official Symbol:
SIK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_079440
Protein GI Number:
38569491
Purification:
SIK3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least three isoforms of SIK3 are known to exist; this antibody will detect two isoforms. SIK3 antibody is predicted to not cross-react with SIK1 and SIK2.
Swissprot #:
Q9Y2K2
User NOte:
Optimal dilutions for each application to be determined by the researcher.