ProSci

GRB10 Antibody

Product Code:
 
PSI-6733
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
1 / 2
Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunofluorescence of GRB10 in rat brain cells with GRB10 antibody at 20 μg/mL.
❮❯

Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of GRB10 in rat brain cells with GRB10 antibody at 20 μg/mL.

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CodeSizePrice
PSI-6733-0.02mg0.02mg£150.00
Quantity:
PSI-6733-0.1mg0.1mg£453.00
Quantity:
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Further Information

Additional Names:
GRB10 Antibody: RSS, IRBP, MEG1, GRB-IR, Grb-10, GRBIR, KIAA0207, Growth factor receptor-bound protein 10, GRB10 adapter protein
Application Note:
GRB10 antibody can be used for detection of GRB10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
GRB10 Antibody: GRB10 belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules and function in diverse cellular processes. GRB10 is a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of GRB10 inhibits tyrosine kinase activity and results in growth suppression. GRB10 can also interact with the L isoform of the proapoptotic protein Bim. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts.
Background References:
  • Morrione A. Grb10 proteins in insulin-like growth factor and insulin receptor signaling (review). Int. J. Mol. Med. 2000; 5:151-4.
  • Liu F and Roth RA. Grb-IR: a SH2-domain-containing protein that binds to the insulin receptor and inhibits its function. Proc. Natl. Acad. Sci. USA 1995; 92:10287-91.
  • Wick KR, Werner ED, Langlais P, et al. Grb10 inhibits insulin-stimulated insulin receptor substrate (IRS)-phosphatidylinositol 3-kinase/Akt signaling pathway by disrupting the association of IRS-1/IRS-2 with the insulin receptor. J. Biol. Chem. 2003; 278:8460-7.
  • Hu ZQ, Zhang JY, Ji CN, et al. Grb10 interacts with Bim L and inhibits apoptosis. Mol. Biol. Rep. 2010; 37:3547-52.
Buffer:
GRB10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%)
Immunogen:
GRB10 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GRB10.

The immunogen is located within amino acids 440 - 490 of GRB10.
NCBI Gene ID #:
2887
NCBI Official Name:
growth factor receptor-bound protein 10
NCBI Official Symbol:
GRB10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005302
Protein GI Number:
48762679
Purification:
GRB10 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
At least three isoforms of GRB10 are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q13322
User NOte:
Optimal dilutions for each application to be determined by the researcher.