Chemodex

7-(Isobutyloxycarbonyloxy)-3H-phenoxazin-3-one

Product Code:
 
CDX-I0005
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
Short term: +4°C. Long term: -20°C
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Chemical Structure
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Chemical Structure

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Further Information

Alternate Names/Synonyms:
7-(Isobutyloxycarbonyloxy)-3H-phenoxazin-3-one; CytoRed
Appearance:
Solid.
CAS:
251292-24-7
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C17H15NO5/c1-10(2)9-21-17(20)22-12-4-6-14-16(8-12)23-15-7-11(19)3-5-13(15)18-14/h3-8,10H,9H2,1-2H3
InChiKey:
RVDFSVVDUVHHKE-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 251292-24-7. Formula: C17H15NO5. MW: 313.3. Synthetic. Cell permeable resorufin derivative used as a fluorogenic indicator for cell viability. Resorufin was selected as the fluorophore because it fluoresces at a longer wavelength and is amenable to modification at the hydroxyl group which is essential for its fluorescence. Incubation with esterase at pH 8.0 results in a 80-90 nm shift of emission max. (Ex/Em: 500/~593nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)). This cell viability indicator is 2 times more sensitive than calcein-AM.
MDL:
MFCD03791103
Molecular Formula:
C17H15NO5
Molecular Weight:
313.3
Package Type:
Vial
Product Description:
Cell permeable resorufin derivative used as a fluorogenic indicator for cell viability. Resorufin was selected as the fluorophore because it fluoresces at a longer wavelength and is amenable to modification at the hydroxyl group which is essential for its fluorescence. Incubation with esterase at pH 8.0 results in a 80-90 nm shift of emission max. (Ex/Em: 500/~593nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)). This cell viability indicator is 2 times more sensitive than calcein-AM.
Purity:
>97% (HPLC)
SMILES:
CC(C)COC(=O)OC1=CC2=C(C=C1)N=C1C=CC(=O)C=C1O2
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.

References

(1) M. Ishiyama et al.; Analyt. Sci. 15, 1025 (1999) | Multiplexing fluorogenic esterase-based viability assay with luciferase assays: K. Ohgane, et al. MethodsX 6, 2013 (2019)