Chemodex

Fluorescein (free acid)

Product Code:
 
CDX-F0023
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
Short term: +20°C. Long term: +20°C
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Chemical Structure
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Chemical Structure

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CDX-F0023-G250250 g£146.00
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CDX-F0023-G500500 g£224.00
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CDX-F0023-K0011 kg£412.00
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Further Information

Alternate Names/Synonyms:
Acid Yellow 73; NSC 667256; 3',6'-Fluorandiol
Appearance:
Red powder.
CAS:
05/07/2321
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS07
Handling Advice:
Keep cool and dry.Protect from light and moisture.
Hazards:
H319
InChi:
InChI=1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
InChiKey:
GNBHRKFJIUUOQI-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 2321-07-5. Formula: C20H12O5. MW: 332.31. Synthetic Fluorescein is a fluorophore commonly used in microscopy, flow cytometry forensics, serology to detect latent blood stains, and in dye tracing (attached to certain biologically active molecules). This fluorescent dye/probe has spectral data of lambdaex 490 nm| lambdaem 514 nm in 0.1 M Tris pH 8.0 and is strongly pH dependent. It has an isosbestic point (equal absorption for all pH values) at 460 nm. Fluorescein derivatives are used in different applications in cell biology.
MDL:
MFCD00005050
Molecular Formula:
C20H12O5
Molecular Weight:
332.31
Package Type:
Vial
Precautions:
P305, P351, P338
Product Description:
Fluorescein is a fluorophore commonly used in microscopy, flow cytometry forensics, serology to detect latent blood stains, and in dye tracing (attached to certain biologically active molecules). This fluorescent dye/probe has spectral data of lambdaex 490 nm| lambdaem 514 nm in 0.1 M Tris pH 8.0 and is strongly pH dependent. It has an isosbestic point (equal absorption for all pH values) at 460 nm. Fluorescein derivatives are used in different applications in cell biology.
Purity:
>99% (HPLC)
Signal word:
Warning
SMILES:
OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(O)C=C1O2
Solubility Chemicals:
Soluble in acetone or DMSO. Insoluble in water or chloroform.
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.

References

(1) R.F. Chen; Arch. Biochem. Biophys. 133, 263 (1969) | (2) R. Sj?back, et al.; Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 51, L7 (1995) | (3) B.L. Drees, et al.; J. Biol. Chem. 271, 32168 (1996) | (4) M. Taki, et al.; Prot. Eng. Des. Select. 17, 119 (2004)