abm’s transfection reagents are unique formulations designed to aid the delivery of nucleic acids into a variety of cell lines with high transfection efficiency and low cell toxicity.
Their collection includes DNAfectin™ Plus for transection into adherent cells, and Susfectin™ for transfection of suspension 293 and CHO cells in the production of recombinant proteins, antibodies, and recombinant viruses.
Products Available:
DNAFectinTM Plus DNA Transfection Reagents
abm’s DNAfectinTM Plus out-performs competitors in transfection efficiency for a variety of popular cell lines. In addition, it has added benefit of performing transfection without the need to remove serum/culture medium.
| PRODUCT | CAT. NO. |
|---|---|
| DNAfectinTM Plus Transfection Reagent* | ABM-G2500 |
| *Serum-compatible | |
Minimal toxicity
Out-performs in a range of cells
Serum compatible
Out-performs competitors
DNAfectinTM Plus out-performs competitors in transfection efficiency for a variety of popular cell lines. DNAfectinTM Plus Transfection Reagent (Cat. No. ABM-G2500) or a competitor’s transfection reagent were used to deliver a GFP construct into CHO-K1, HeLa, HEK-293T or Calu-6 cells. Images were taken 24 hours post-transfection.
No medium changes needed
DNAfectinTM Plus enables transfection in the presence of serum. HEK-293T Cells were transfected with a GFP construct using DNAfectin™ Plus (Cat. No. ABM-G2500) or a competitor’s transfection reagent. DNAfectin™ Plus does not require removal of serum/culture medium during the transfection, as the transfection mixture can be added directly to complete medium.
SusfectinTM Suspension Cell Transfection Reagent
abm’s SusfectinTM Transfection Reagent is uniquely formulated for transfection of DNA into eukaryotic suspension cells without the need to remove serum/culture medium. This simplified workflow makes SusfectinTM the best choice for transfection of CHO and 293 cells.
| PRODUCT | CAT. NO. |
|---|---|
| Susfectin™ Transfection Reagent* | ABM-G4000 |
| *Serum-compatible | |
Best choice for suspension cells (CHO, 293)
Minimal toxicity
Serum compatible
Excellent for suspension cells
abm’s Susfectin™ Transfection Reagent enables high efficiency transfection of suspension cells. HEK293 suspension cells was transfected with a Scrambled siRNA GFP Lentivector (Cat. No. ABM-LV015-G) using Susfectin™ Transfection Reagent (Cat. No. ABM-G4000).
2X higher expression
Susfectin™ results in 2X higher expression compared to PEI. CHO cells were transfected using Susfectin™ (Cat. No. ABM-G4000), a competing transfection reagent, or linear PEI. Recombinant antibody levels in the supernatant were quantified using ELISA.
Information originally posted on: https://www.abmgood.com/Transfection-Reagent-Protocol-Efficiency.html
Caltag Medsystems is the distributor of abm products in the UK and Ireland. If you have any questions about these products, please contact us.