Osteoblast Mineralization Medium

Complete kit (includes supplements)

Osteoblast Mineralization Medium (ObMM), when used with Osteoblast Mineralization Supplement (ObMS, Cat #4672), and 25 ml of fetal bovine serum (FBS) is a complete medium designed for the maturation of normal osteoblasts in vitro . It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively promotes maturation and mineralization of normal osteoblasts in vitro .

ObMM consists of 500 ml of basal medium, 25 ml of FBS (Cat #0025), 5 ml of Osteoblast Mineralization Supplement (ObMS, Cat #4672), and 5 ml of penicillin/streptomycin solution (P/S, Cat #0503). Note: FBS, ObMS, and P/S are not pre-mixed in ObMM; they must be added separately to make the complete ObMM.