Applied Biological Materials (abm)

Immortalized Human Peripheral Blood CD4+ Helper T Cell Line - SV40

Product Code:
 
ABM-T0417
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

No additional charges, what you see is what you pay! *

CodeSizePrice
ABM-T04171x10^6 cells / 1.0 mlEnquire
An MTA must be signed before products can be dispatched. Please contact us for more information.
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: Canada.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Related Products
  • Show All

Further Information

Description:
This cell line is derived from human peripheral blood CD4+ helper T cells, which are crucial for orchestrating immune responses. Cells were immortalized via the introduction of the SV40 (Simian Virus 40) large T antigen. This modification allows for extensive experimental manipulation and long-term studies on CD4+ T cell functions and mechanisms. Researchers can utilize this cell line to investigate various aspects of immune regulation, including T cell activation, cytokine production, and interactions with other immune cells, providing valuable insights into immune system diseases and potential therapeutic targets.
Donor History:
Male, 28, Caucasian
Growth Conditions:
PriCoat™ T25 Flasks  (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow X Series Medium for T0417 (TM0417) + 25 ul of Immunocult Human CD3/CD28 T Cell Activator (Stemcell, Cat.10971) / 106 T cells. Note: Once cells recover from freeze-thaw conditions, the T cell activator does not need to be added to the culture vessel. 
Growth Properties:
Suspension
Immortalisation Method:
Serial passaging and retroviral transduction with retroviruses carrying the SV40 gene.
Markers:
CD4+
Tissue:
Blood (Immune)