Applied Biological Materials (abm)

Immortalized Normal Human Oral Mucosal Keratinocytes

Product Code:
 
ABM-T0768
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

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ABM-T07685x10? cells / 1.0 mlEnquire
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Further Information

Description:
Immortalized Normal Human Oral Mucosal Keratinocytes (OMK) were isolated from human buccal mucosa. This cell line was immortalized using a vector carrying HPV16 E6/E7 genes. Cell lysates of immortalized OMKs are reliable substrates for the detection of mucous membrane pemphigoid autoantigens.
Donor History:
Female, 34
Growth Conditions:
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0768) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2
Growth Properties:
Adherent, epithelial
Immortalisation Method:
Immortalized with lentiviral vector carrying HPV16 E6/E7 genes (pLVSIN-EF1-HPV16-E6_7-Pur)
Population Doubling:
24 - 34
Seeding Density (/cm^2):
10,000 - 20,000
Tissue:
Mouth/Oral

References

Kamaguchi, M., Iwata, H., Miyauchi, T., Ujiie, H., Ujiie, I., Nomura, T., Ohga, N., Shimizu, H., & Kitagawa, Y. (2019). The identification of autoantigens in mucous membrane pemphigoid using immortalized oral mucosal keratinocytes. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 48(1), 60?67. https://doi.org/10.1111/jop.12780