Applied Biological Materials (abm)

Immortalized Mouse Fetal Hypothalamic Neural Stem Cells (AC1)

Product Code:
 
ABM-T0861
Product Group:
 
Cell Lines
Host Type:
 
Mouse
Regulatory Status:
 
RUO
 

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CodeSizePrice
ABM-T08611x10^6 cells / 1.0 mlEnquire
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Further Information

Description:
Cells were derived from hypothalamic region of E14.5 mouse brain and has been propagated for at least 25 passages (more than 100 duplications). The cells maintain high neurogenic potential and can generate neuronal cells expressing some hypothalamic peptides. Useful tool to investigate in vitro the mechanisms possibly involved in development, differentiation, and fate of hypothalamic neurons with neuroendocrine features.
Donor History:
E14.5 mouse
Growth Conditions:
Laminin coating is recommended for cell adhesion to the culture vessel. PriGrow X Series Medium (TM0861) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2. Thaw in T25, detach cells through gentle pipetting.
Growth Properties:
Adherent, glia-like
Immortalisation Method:
Spontaneously immortalized
Markers:
Arnt2 and Mash1 (Immunofluorescence); Nestin, Sox2, and Pax6 (RT-PCR)
Population Doubling:
24
Split Ratio:
1:2 to 1:4
Tissue:
Brain

References

Cariboni A, Conti L, Andr? V, Aprile D, Zasso J, Maggi R. Establishment of a radial glia-like mouse fetal hypothalamic neural stem cell line (AC1) able to differentiate into neuroendocrine cells. Neurogenesis (Austin). 2014 Jul 28;1(1):e29950. doi: 10.4161/neur.29950. PMID: 28255570; PMCID: PMC5322806.