Applied Biological Materials (abm)

Immortalized Human Oral Gingival Keratinocytes (HPV-16 GM)

Product Code:
 
ABM-T0717
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

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ABM-T07171x10^6 cells / 1.0 mlEnquire
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Further Information

Description:
Immortalized Human Oral Gingival Keratinocytes (HPV-16 GM) are derived from a healthy donor and were immortalized using HPV16 E6/E7 genes. Immortalized Human Oral Gingival Keratinocytes (HPV-16 GM) express the following typical epithelial marker genes: cytokeratin 1, cytokeratin 10, involucrin, and filaggrin. Characterization by indirect immunofluorescence, RT PCR, and transplantation assay.
Donor History:
Healthy donor
Growth Conditions:
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0717), 37.0°C, 5% CO2
Growth Properties:
Adherent, cobblestone
Immortalisation Method:
Immortalized with retrovirus containing the E6/E7 genes of HPV16
Markers:
Cytokeratin 1, cytokeratin 10, involucrin, and filaggrin
Population Doubling:
25 - 35
Seeding Density (/cm^2):
20,000 - 40,000
Split Ratio:
1:2 to 1:5
Tissue:
Mouth/Oral

References

Roesch-Ely, M., Steinberg, T., Xavier Bosch, F., M?ssig, E., Whitaker, N., Wiest, T., ? Tomakidi, P. (2006). Organotypic co-cultures allow for immortalized human gingival keratinocytes to reconstitute a gingival epithelial phenotype in vitro. Differentiation, 74(9?10), 622?637. https://doi.org/10.1111/j.1432-0436.2006.00099.x