Applied Biological Materials (abm)

Immortalized Human T Cells

Product Code:
 
ABM-T0086
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

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CodeSizePrice
ABM-T00861x10^6 cells / 1.0 mlEnquire
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Further Information

Description:
The Immortalized Human T Cells are genetically modified to proliferate indefinitely while retaining their functional characteristics, making them an invaluable tool for immunological research and therapeutic development. These cells offer a consistent and reliable model for studying T cell biology, including mechanisms of activation, signaling pathways, and immune responses. Additionally, they are widely used in preclinical studies for developing and testing novel immunotherapies, including CAR-T cell therapies and vaccines. Their ability to be expanded in vitro without senescence allows for large-scale production and in-depth functional assays, facilitating advancements in personalized medicine and immunotherapy research.
Donor History:
Healthy donor, no other details disclosed
Growth Conditions:
T12.5 flasks are recommended for optimal cell culture. PriGrow X Series Medium (TM0086) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2.  Immunocult Human CD3/CD28 T Cell Activator (Stemcell, Cat. 10971) is required for T cell activation upon thawing. Note: refer to the detailed "Thawing Protocol" and "Subculturing Protocol" sections of the Datasheet for complete instructions regarding handling cells. Do not attempt to work with cells until these sections have been reviewed and understood. The post-thaw viability of T0086 can be low, however, correct handling, as per the protocols below, will aid in cell recovery. Cells should form clumps once activated. We recommend thawing frozen cells in T12.5 cell culture flasks. Do not grow cells in culture vessels exceeding a surface area of 25cm2.
Growth Properties:
Suspension, round; cells grow as round clumps
Immortalisation Method:
Transduction with Lenti-hTERT (LV616) lentivirus and Lenti-Rb siRNA (LV621) lentivirus.
Markers:
CD3 and HLA-27 (via FACS)
Tissue:
Blood