Human Induced Neural Stem Cells (HiNSC-DLK)
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Further Information
A key feature of these Human Induced Neural Stem Cells (HiNSC-DLK) is their robust differentiation into TUJ1-positive neurons within just four days, irrespective of the media composition. This rapid differentiation makes them ideal for use in innervated co-cultures. Furthemore, cells demonstrate the ability to migrate, engraft, and contribute to both the central and peripheral nervous systems thereby making them a useful 3D human brain model.
hiNSC cultures should be grown on a feeder layer of mouse embryonic fibroblasts (MEFs) inactivated by Mitomycin C for three hours. PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow X Series Medium for T0441 (TM0441) + 20% TM0441-S + 1% Glutamax + 0.1 mM β-mercaptoethanol + 1% Penicillin/Streptomycin Solution (G255) + 20ng/mL Basic Fibroblast Growth Factor (spike 50mL aliquots day of/day before use), 37.0°C, 5% CO2.Note: Allow feeder layer to become 90-100% confluent prior to inactivation; attachment of cells improves with a more confluent feeder layer. The population doubling time for this cell line is 4-7 days which can extend to 14 days in certain cases. As long colony formation and adherence is noted in the cell line, cells are viable. After 14 days, MEFs will detach from surface.
Adherent, spherical clumps that attach to feeder layer
OCT4, SOX2, NANOG, SSEA4, TRA-1-81, PAX6, SOX1, NESTIN, CD133, βIII-tubulin (TUJ1), glial fibrillary acidic protein (GFAP), GEPHYRIN, VGAT, PSD95, VGLUT1, SYNAPTOPHYSIN, PAN-NAV, S100β, O4, MBP.
4-7 days (up to 14 days; past 14 days MEFs will detach from plate)
Brain
