Applied Biological Materials (abm)

Alkaline Phosphatase Conjugated Affinity Purified anti-Sheep IgG [H&L] [Donkey]

Product Code:
 
ABM-SA029
Product Group:
 
Secondary Antibody
Host Type:
 
Donkey
Antibody Clonality:
 
Secondary Antibody
Regulatory Status:
 
RUO
Target Species:
 
Sheep
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoblotting
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice Pack
Storage:
 
Store vial at 4°C before opening. DO NOT FREEZE. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
 

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CodeSizePrice
ABM-SA029250 ug£227.00
Quantity:
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This product comes from: Canada.
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Further Information

Application Note:
This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:14,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Concentration:
1.0 mg/ml (by UV absorbance at 280 nm)
Description:
Minimum Cross Reactivity to Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins
Immunogen:
Sheep IgG, whole molecule
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
Storage Buffer:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0