Epithelial Cell Medium

Epithelial Cell Medium (EpiCM) is a complete medium designed for optimal growth of normal human epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human epithelial cells in vitro.

EpiCM consists of 500 ml of basal medium, 10 ml of fetal bovine serum (FBS, Cat. No. 0010), 5 ml of epithelial cell growth supplement (EpiCGS, Cat. No. 4152), and 5 ml of Antibiotic Solution (P/S, Cat. No. 0503).