Schwann Cell Medium

Schwann Cell Medium (SCM), when used with Schwann Cell Growth Supplement (SCGS, Cat #1752) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal schwann cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal schwann cells in vitro.

SCM consists of 500 ml of basal medium, 25 ml of FBS (Cat. #0025), 5 ml of Schwann Cell Growth Supplement (SCGS, Cat. #1752) and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503). Note: FBS, SCGS and P/S are not pre-mixed in SCM; they must be added separately to make the complete SCM.