Osteoblast Medium

Osteoblast Medium (ObM), when used with Osteoblast Growth Supplement (ObGS, Cat #4652) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal osteoblasts in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal osteoblasts in vitro.

ObM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of osteoblast growth supplement (ObGS, Cat. No. 4652) and 5 ml of Antibiotic solution (P/S, Cat. No. 0503).