Mouse Embryonic Fibroblast-Conditioned Medium
Product Code:
SC-5881
SC-5881
Regulatory Status:
RUO
RUO
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Dry Ice
Dry Ice
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| Code | Size | Price |
|---|
| SC-5881 | 100 ml | £220.00 |
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Typical lead time: 10-14 working days.
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Further Information
Description:
Mouse Embryonic Fibroblast-Conditioned Medium (MEF-cm) is a serum free medium for the feeder independent culture of human pluripotent stem cells (hPSCs), including embryonic stem cells and induced pluripotent stem cells. MEF-cm is an efficient alternative to MEF feeder cells, supporting the self-renewal of hPSC and maintaining pluripotency in the presence of basic FGF and hPSC qualified matrix (e.g., BD MatrigelTM matrix). ScienCell's MEF-cm was prepared using high quality mitomycin C treated MEF (MEF-mt, Cat. #M7570-mt) derived from E13 embryos of CF1 mice. Serum free medium was conditioned with mitomycin C treated CF1 MEFs for 24 hours. The conditioned medium was collected daily for 7 days, pooled and sterilized by filtering through a 0.22 um filter. Before using MEF-cm for hPSC culture, please add fresh recombinant human basic FGF (4 ng/mL) (rhbFGF, Cat #104-02) into the medium to make the complete medium. The complete MEF-cm has been tested for its ability to support hPSC self-renewal on BD MatrigelTM matrix in vitro. The medium color of each lot may vary but does not affect performance.
Extra Description:
MEF-cm is a liquid medium prepared from primary CF1 mouse embryonic fibroblasts treated with mitomycin C. The medium is sterile filtered and consists of one 100-mL bottle.