Human Esophageal Fibroblasts

HEsF from ScienCell Research Laboratories are isolated from human esophageal tissue. HEsF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume.

Esophageal fibroblasts, which line the lamina propria of the upper and lower esophagus, are mesenchymal cells derived from the embryonic mesoderm. Fibroblasts secrete a non-rigid extracellular matrix that is both rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play a central role in wound healing. They have been extensively used for a wide range of cellular and molecular studies, as they are one of the easiest types of cells to grow in culture. In addition, their durability makes them amenable to a variety of manipulations ranging from gene transfection to microinjection. Esophageal fibroblasts have been shown to contribute to the esophageal squamous cell carcinoma-induced angiogenesis through the TGF-β and VEGF signaling pathways. HEsF from ScienCell Research Laboratories are isolated from human esophageal tissue. HEsF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HEsF are characterized by immunofluorescence with antibody specific to fibronectin. HEsF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEsF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium - It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HEsF in vitro .