Sciencell

Mouse Cardiac Fibroblasts from C57BL/6

Product Code:
SC-M6300-57
Product Group:
Primary Cells
Supplier:
Sciencell
Cell System:
Cardiovascular Cell System
Cell Type:
Fibroblasts
Species:
Mouse
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry ice.
Storage:
Upon receiving directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Extra Description:
Isolated from postnatal day 2 or postnatal day 8 C57BL/6 mouse heart. MCF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume.
Long Description:
The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes, are a source of paracrine growth factors, and are an important cellular component of myocardial responses to injury. CF are responsible for extracellular matrix synthesis in the heart during development and pathological conditions. Their proliferation and matrix synthesis is essential for scar formation at sites of myocardial infarction, cardiac fibrosis, and is often complicated by cardiac hypertrophy. CF also respond to changes in microenvironments in pathological conditions by modulating integrin expression. CF culture has been widely used as a model to study the cardiac matrix remodeling by physiological (exercise) and pathological (hypertension) stressors. MCF from ScienCell Research Laboratories are isolated from postnatal day 2 or postnatal day 8 C57BL/6 mouse heart. MCF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MCF are characterized by immunofluorescence with antibody specific to fibronectin. MCF are negative for mycoplasma, bacteria, yeast, and fungi. MCF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium - It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing MCF in vitro .