DB RT-PCR Mixes
In a customer study, the relative expression of two Arabidopsis thaliana genes was analysed under different light conditions. Quantitative RT-PCR was performed using DIANA’s DB RT-PCR SYBR Mix and a leading competitor mix. Melting curve analysis revealed multiple non-specific products for each target with the competitor mix, sometimes completely failing to amplify the specific product. In contrast, DB RT-PCR SYBR Mix produced a single, specific amplicon for each target. The high specificity of DIANA’s mixes simplifies primer design and ensures reliable quantification across experiments.
DBdirect™ RT-PCR Mixes
CCRF-CEM human cells were FACS-sorted directly into PCR plates containing 10 μL of RT-PCR mix and processed by one-step RT-qPCR.
Two transcripts (GAPDH and TBP) were detected using either sequence-specific TaqMan probes or SYBR Green I dye.
DBdirect RT-PCR mixes enabled precise mRNA quantification from single cells without RNA extraction, allowing rapid, cost-efficient RNA profiling at single-cell resolution.
Information provided by DIANA Biotechnologies.
Caltag is the distributor of DIANA Biotechnologies’ products in the UK and Ireland. If you have any questions about these products, please contact us.