PCR Polymerases

abm’s pursuit of next generation PCR enzymes has led to intensive R&D and significant upgrades to their PCR products. The result – a new collection of world leading PCR enzymes and formulations with performance far beyond the current leading brands.

Products Available:

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PCR Sure™ Kit

Simplify the PCR optimisation process with our PCR Sure™ Kit. With a system consisting of multiple thermostable DNA polymerases pre-mixed with optimised buffers, your PCR is guaranteed to work!

PRODUCT	CAT. NO.
PCR Sure™ Kit	ABM-G065

Super Versatile

Amplify your difficult templates with abm’s PCR Sure™ Kit. From crude templates to purified plasmids, High GC to Low GC, human, mouse, rat or plant DNA, one of their highly optimised mastermixes is sure to work!

Extremely Easy to Use

With 8 optimised 2X MasterMixes, simply add your primers and template and run on one single program!

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MegaFi™ Pro Fidelity DNA Polymerase

MegaFi™ Pro Fidelity DNA Polymerase is the best high fidelity DNA Polymerase on the market. With 2000X better fidelity than regular Taq, this ultrasensitive polymerase amplifies even the most difficult templates.

PRODUCT	CAT. NO.
MegaFi™ Pro Fidelity DNA Polymerase	ABM-G886
MegaFi™ Fidelity 2X PCR MasterMix	ABM-G887
MegaFi™ Pro One-Step RT-PCR *	ABM-G597
*Contains OneScript™ Hot Reverse Transcriptase (Cat. No. ABM-G593)

Ultra-Low Error Rates

MegaFi™ Pro DNA Polymerase is THE BEST high fidelity DNA polymerase A lacZa-containing sequence was amplified using various DNA polymerases, subcloned, and plated on X-gal-containing media. Colonies without PCR amplified lacZa mutations appear dark blue, while those that have PCR introduced frameshift mutations appear white.

Superior Fidelity

MegaFi™ Pro Fidelity DNA Polymerase has ~2000X higher fidelity than other enzymes on the market. abm’s MegaFi™ Pro Fidelity DNA Polymerase (Cat. No. ABM-G886) has the highest accuracy rate compared to other DNA polymerases as tested using blue/white colony screening. Shown as relative fidelity compared to Taq DNA Polymerase (Taq = 1X). PCR cycle number and mutations resulting in non-phenotypic changes are accounted for.

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Kodaq 2X PCR MasterMix

Kodaq 2X PCR MasterMix has a ready-to-use formula which includes a uniquely-formulated buffer with gel loading dye and strategically engineered mutations for robust, high-fidelity performance. The buffer accommodates high AT and GC content and common PCR inhibitors found in DNA samples.

PRODUCT	CAT. NO.
Kodaq 2X PCR MasterMix	ABM-G883

Highly Robust

PCR amplification of a GC-Rich 1.4 kbp plasmid template using abm’s Kodaq 2X PCR MasterMix (Cat. No. ABM-G883) (column 1) vs. competitor products (column 2 to 5).

PCR amplification of difficult templates (including plant gDNA) using abm’s Kodaq 2X PCR MasterMix (Cat. No. ABM-G883) (column 1) vs. competitor products (column 2 and 3). The red arrows indicate the expected product sizes.

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BlasTaq™ DNA Polymerase

Blast through a routine 2 hour PCR run in only 20 minutes with abm’s BlasTaq™ DNA Polymerase. Reach extension speeds as fast as 6 kb/min and streamline your PCR!

PRODUCT	CAT. NO.
BlasTaq™ DNA Polymerase	ABM-G894
BlasTaq™ 2X PCR MasterMix	ABM-G895

Super Fast Extension Speeds

BlasTaq™ DNA Polymerase enables extension speeds as fast as 6 kb/min. Total reaction times for abm’s BlasTaq™ DNA Polymerase (Cat. No. ABM-G894) and Taq were determined for the amplification of different sized amplicons: 500 bp, 1 kb, 2 kb, and 5 kb. Reaction times are based on a 30-cycle program using the recommended reaction protocol for each enzyme.

Highly Robust

PCR amplification using abm’s BlasTaq™ DNA Polymerase (Cat. No. ABM-G894) (column 1) of various targets vs. competitor polymerases (column 2 and 3) followed by electrophoresis on a 1% agarose gel. A) 1 kb target from Human cDNA. B) 1.3 kb target from plasmid DNA. C) 7.6 kb target from plasmid DNA.

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BlasTaq™ HotStart DNA Polymerase

Eliminate non-specific amplification and primer-dimer formation with abm’s next generation BlasTaq™ HotStart DNA Polymerase. This formulation includes a proprietary antibody that blocks polymerase activity at low temperatures and dissociates at the denaturation step to restore enzyme activity.

PRODUCT	CAT. NO.
BlasTaq™ HotStart DNA Polymerase	ABM-G595
BlasTaq™ HotStart 2X PCR MasterMix	ABM-G598

Excellent for suspension cells

BlasTaq™ HotStart DNA Polymerase eliminates non-specific amplification. abm’s BlasTaq™ HotStart DNA Polymerase (Cat. No. ABM-G595) was used to amplify a 7 kb target. The antibody concentration was decreased incrementally from lanes 1-5. Lane 1 is their BlasTaq™ HotStart formulation and lane 5 contains no antibody.

Super Fast Extension Speeds

BlasTaq™ HotStart DNA Polymerase has extension speeds of 6 kb/min. abm’s BlasTaq™ HotStart DNA Polymerase (Cat. No. ABM-G595) was used to amplify different sized amplicons (500 bp, 1 kb, 2 kb, and 5 kb). Total reaction times are listed based on a 30-cycle program using the recommended reaction protocol for each enzyme.

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Ultra-Pure BlasTaq™ 2X PCR MasterMix

Take your PCR to the next level with Ultra-Pure BlasTaq™ 2X PCR MasterMix. This formulation includes the same strategically-engineered, next generation Taq Polymerase which was subject to rigorous multi-step purification protocol using physical, chemical, and enzymatic methods for maximum removal of contaminating genomic DNA.

PRODUCT	CAT. NO.
Ultra-Pure BlasTaq™ 2X PCR MasterMix	ABM-G885

High Purity

Ultra-Pure BlasTaq™ PCR MasterMix ensures efficient amplification while maintaining low-DNA contamination levels when compared to leading competitors. Two different primer sets (blue and orange) against host DNA were tested with two competitor products (1 and 2) and abm’s Ultra-Pure BlasTaq™ enzyme (3). There is no amplification seen in the no-template controls (NTC) from Ultra-Pure BlasTaq™ PCR MasterMix indicating exceptional purity of the product. Activity Control Primer Set demonstrated similar performance for all three tested products.

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Unsure which polymerase is right for you?

CHARACTERISTIC	MegaFi™ Pro Fidelity	BlasTaq™	BlasTaq™ HotStart	Ultra-Pure BlasTaq™	Taq	Kodaq
Proofreading	Yes	No	No	No	No	Yes
Fidelity (vs. Native Taq)	1300X	1X	1X	1X	1X	50X
Specificity	••••	•••	••••	•••	•••	••••
Extension Speed	20-30 sec/kb	15 sec/kb	15 sec/kb	15 sec/kb	60 sec/kb	60 sec/kb
Max Target Length	15-20 kb	10-15 kb	10-15 kb	10-15 kb	6.5 kb	15-20 kb
Suitable for TA cloning	No	Yes	Yes	Yes	Yes	No
Special Feature	Fidelity and Sensitivity	Fast and Robust	High Specificity	High Purity	For routine PCR	Fidelity and Highly Robust

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Information originally posted on: https://www.abmgood.com/Taq-Polymerase.html

Caltag Medsystems is the distributor of abm products in the UK and Ireland. If you have any questions about these products, please contact us.