AdipoGen Life Sciences

anti-alpha-Tubulin mAb (rec.) (F2C) (ATTO488)

Product Code:
 
AG-27B-0005TD
Product Group:
 
Primary Antibodies
Host Type:
 
Human
Antibody Isotype:
 
IgG2λ
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
F2C
Regulatory Status:
 
RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Mouse
Application:
 
Immunocytochemistry (ICC)
Shipping:
 
Blue Ice
Storage:
 
Short term: +4°C. Long term: +4°C
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Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.) (F2C) (ATTO 488) (Prod. No. AG-27B-0005TD). Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-
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Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.) (F2C) (ATTO 488) (Prod. No. AG-27B-0005TD). Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-

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AG-27B-0005TD-C100100 ug£301.00
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Further Information

Concentration:
Lot-dependent
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 0.02% sodium azide.
Immunogen:
Bovine brain tubulin.
Labels - Conjugates:
ATTO 488
Long Description:
Recombinant Antibody. Recognizes mouse, bovine and human alpha-tubulin. Species cross-reactivity: Bovine, Human, Mouse. Clone: F2C. Isotype: Human IgG2lambda. Applications: ICC. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 0.02% sodium azide. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.
NCBI, Uniprot Number:
N/A
Other data:
anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) (ATTO488) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) (ATTO488) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Package Type:
Plastic Vial
Product Description:
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.
Purity:
>95% (SDS-PAGE)
Source / Host:
Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Specificity:
Recognizes mouse, bovine and human alpha-tubulin.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibody Conjugates
UNSPSC Number:
12352203
Use & Stability:
Keep conjugated formats at +4°C. Stable for at least 1 year after receipt when stored at +4°C.

References

Recombinant antibodies selected against subcellular fractions to track endogenous protein dynamics in vivo: C. Nizak, et al.; Traffic 7, 739 (2003) | Characterization of single chain antibody targets through yeast two hybrid: O. Vielemeyer, et al; BMC Biotechnol. 10, 59 (2010)