TIM-3 Antibody [3G7]

Product Code:

PSI-RF16105

Product Group:

Primary Antibodies

Supplier:

ProSci

Host Type:

Mouse

Antibody Isotype:

IgG1,k

Antibody Clonality:

Monoclonal

Antibody Clone:

3G7

Regulatory Status:

RUO

Target Species:

Human

Applications:

Immunocytochemistry (ICC)
Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)

Shipping:

blue ice

Storage:

TIM-3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

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Code	Size	Price

PSI-RF16105-0.02mg

0.02mg

£167.00

Quantity:

PSI-RF16105-0.1mg

0.1mg

£469.00

Quantity:

Prices exclude any Taxes / VAT

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Further Information

Additional Names:

TIM-3 Antibody: Hepatitis A virus cellular receptor, HAVCR2, TIM3, CD366, KIM-3, TIMD3, TIMD-3

Application Note:

TIM-3 antibody can be used for ELISA starting at 0.1 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL. For Flow Cytometry start at 0.1 μg/ml.

Background:

The immune checkpoint protein TIM3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-? producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).

Background References:

Monney L, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of autoimmune disease. Nature 2002; 415:536-41.
Sakuishi K, Apetoh L, Sullivan JM, et al. Targeting Tim-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tumor immunity. J Exp Med 2010; 207:2187?94.
Zhou Q, Munger ME, Veenstra RG, et al. Coexpression of Tim-3 and PD-1 identifies a CD8+ T-cell exhaustion phenotype in mice with disseminated acute myelogenous leukemia. Blood 2011; 117:4501?10.

Buffer:

TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

TIM-3 antibody was raised against the extracellular domain of human TIM-3

NCBI Gene ID #:

84868

NCBI Official Name:

hepatitis A virus cellular receptor 2

NCBI Official Symbol:

HAVCR2

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

NP_116171

Protein GI Number:

84868

Purification:

TIM-3 Antibody is supplied as protein A purified IgG1.

Research Area:

Cell Cycle,Cancer,Immunology

Swissprot #:

Q8TDQ0

User NOte:

Optimal dilutions for each application to be determined by the researcher.

TIM-3 Antibody [3G7]

Further Information

Documents