Applied Biological Materials (abm)

Immortalized Human Epidermal Keratinocytes

Product Code:
 
ABM-T0345
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

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CodeSizePrice
ABM-T03451x10^6 cells / 1.0 mlEnquire
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Further Information

Description:
Real Time PCR was used to quantify the transgene expression
Donor History:
Not disclosed
Growth Conditions:
PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Keratinocyte Medium Kit (TM040), 37.0°C, 5% CO2.Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. 
Growth Properties:
Adherent, polygonal
Immortalisation Method:
Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Markers:
K1, Puromycin and Neomycin-resistance
Population Doubling:
24 - 34
Seeding Density (/cm^2):
10,000 - 15,000
Split Ratio:
1:2 to 1:3
Tissue:
Skin