Empower Your Chondrocyte Research

Introducing CELLvo™ Human Chondrocytes and CELLvo™ Matrix, A New Cell Culture System

Young, healthy cells at early passage, or older, uncultured osteoarthritic cells – your choice. We offer the means for a deeper understanding of authentic chondrocyte behaviour.

Broaden your knowledge by comparing healthy and diseased chondrocytes, or expand chondrocyte cultures while maintaining potency and natural phenotype with reduced dedifferentiation.

When you grow CELLvo™ Human Chondrocyte cells on CELLvo™ Matrix, you have the ability to truly empower your research.

What You Need To Empower Your Chondrocyte Research:

Uncultured Or Low Passage Human Chondrocytes

CELLvo™ Human Chondrocytes (<40y) are mature, primary cells sourced from young individuals. They may be selected as uncultured (P0), or isolated and expanded on CELLvo™ Matrix.

CELLvo™ Healthy Human Chondrocytes (>50) are primary cells, sourced from healthy individuals 50 to 70-years old and verified free of osteoarthritis. Cells are supplied as uncultured (P0).

CELLvo™ Osteoarthritic Human Chondrocytes (>50) are primary cells, sourced from osteoarthritic individuals 50 to 70-years old. Cells are supplied as uncultured (P0).

A Growth Substrate For Rapid Chondrocyte Expansion

CELLvo™ Matrix is an Extracellular Matrix providing unique support for chondrocyte expansion. When grown on this matrix, CELLvo™ Human Chondrocytes attach and proliferate more rapidly, and exhibit similar morphology to in vivo chondrocytes. CELLvo™ Matrix is composed of more than 150 proteins that were secreted and assembled by bone marrow cells. The final product is deposited on the surface of a culture vessel. Cells are then gently extracted leaving a a native three-dimensional microenvironment providing for rapid and reliable expansion of cells.


Frequently Asked Questions:

If you can’t find an answer to your question, call us on 01280 827460 or send us an e-mail, we’re be happy to help!

Q: Are CELLvo™ Human Chondrocytes better than most commercially available Chondrocytes?

Yes, CELLvo™ Human Chondrocytes are sourced from rigorously defined donor tissue specified by age and health status. Our Human Chondrocytes are gently processed and provided at P0 or at P1. You will always receive cells that are closer to naturally occurring chondrocytes than any other cell available.

Q: How do CELLvo™ Chondrocytes proliferate faster?

When isolated and expanded using CELLvo™ Matrix, the cells proliferate and expand more naturally. In the chart below, data show a marked difference in P0 and P1 cells cultured on CELLvo™ Matrix and tissue cultured polystyrene:

Cell expansion on different substrates for a set length of time. (A) P0 cells were cultured on CELLvo™ Matrix and tissue culture polystyrene for 8 days, and cell numbers were counted. (B) P1 cells were cultured on CELLvo™ Matrix and tissue culture polystyrene for 6 days, and cell numbers were counted. Data are presented as mean ± SD (n  3, ***p &lt; 0.005)

Cell expansion on different substrates for a set length of time. (A) P0 cells were cultured on CELLvo™ Matrix and tissue culture polystyrene for 8 days, and cell numbers were counted. (B) P1 cells were cultured on CELLvo™ Matrix and tissue culture polystyrene for 6 days, and cell numbers were counted. Data are presented as mean ± SD (n  3, ***p < 0.005)

Q: How do CELLvo™ Human Chondrocytes impact my research study?

In two ways: By maintaining natural phenotype as shown by the comparison of COL2A1/COL1A1 ratio in the chart below, and, in the case of CELLvo™ Osteoarthritic and Healthy Chondrocytes, improved experimental design for cartilage therapies and regenerative medicine.

Dedifferentiation is characterized by an increased expression of fibroblast marker (type I collagen) and a decreased expression of chondrocyte markers (type II collagen and aggrecan)Relative gene expression in HAC cultured on CELLvo™ Matrix and tissue culture polystyrene for a similar PDL. The ratios of COL2A1/COL1A1 of P0 cells were calculated as described in the Materials and methods section. Data are presented as mean ± SD (n = 4, *p &lt; 0.05, **p &lt; 0.01 and ***p &lt; 0.005).

Dedifferentiation is characterized by an increased expression of fibroblast marker (type I collagen) and a decreased expression of chondrocyte markers (type II collagen and aggrecan)Relative gene expression in HAC cultured on CELLvo™ Matrix and tissue culture polystyrene for a similar PDL. The ratios of COL2A1/COL1A1 of P0 cells were calculated as described in the Materials and methods section. Data are presented as mean ± SD (n = 4, *p < 0.05, **p < 0.01 and ***p < 0.005).

Q: Why culture my Chondrocytes on CELLvo™ Matrix versus other comparable products?

Because chondrocytes cultured on the CELLvo™ Matrix are more responsive to stimuli, and are not “busy” responding to a foreign microenvironment. CELLvo™ Matrix is a naturally produced biomimetic substrate that allows chondrocytes to respond to experimental variables rather than an artificial substrate.

Q: Are CELLvo™ Human Chondrocytes and CELLvo™ Matrix produced under GMP guidelines?

The StemBioSys facility was built with GMP certification in mind. We have two different areas; One area is for making reagents used in the CELLvo™ Matrix process and preparing healthy donor cells for the process, and the second area is a Class 10,000 (ISO 7) room with HEPA filtered air and monitored positive pressure. The CELLvo™ Matrix process is performed in Class 100 (ISO 5) biological safety cabinets within the clean room. While our current product is for research use only and does not require GMP, we are manufacturing the product under the spirit of GMP requirements in anticipation that we will switch to full GMP certification in the near future.

Learn More…

CELLvo™ Human Chondrocytes are the only cells isolated and expanded using StemBioSys’ CELLvo™ Matrix technology. Our process allows us to preserve more natural phenotypes. All of our cells are early-passage and xeno-free whenever possible.

 

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Empower Your Chondrocyte Research