TargetMol

TEV Protease Protein, TEV, Recombinant (His)

Product Code:
 
TAR-TMPS-00131
Product Group:
 
Recombinant Proteins
Supplier:
 
TargetMol
Regulatory Status:
 
RUO
Application:
 
SDS-PAGE
Shipping:
 
Cool pack
Storage:
 
-20°C
1 / 1
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CodeSizePrice
TAR-TMPS-00131-1000U1000 UEnquire
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TAR-TMPS-00131-10000U10000 UEnquire
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This product comes from: Austria.
Typical lead time: 10-14 working days.
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Further Information

Bioactivity:
Tobacco Etch Virus (TEV) protease is a highly site-specific cysteine protease commonly used to remove affinity tags from fusion proteins. It recognizes the sequence Glu-Asn-Leu-Tyr-Phe-Gln&8595;Gly/Ser (ENLYFQ&8595;G/S), with cleavage occurring between the Gln and Gly or Ser residue. The most frequently used recognition site is ENLYFQG. The optimal cleavage temperature is 30°C, though conditions such as enzyme amount, incubation time, and temperature may require optimization for different substrates. TEV protease includes an N-terminal 6xHis tag, allowing for convenient removal after digestion via Ni2+ affinity chromatography. The recombinant enzyme consists of 231 amino acids, has a molecular mass of approximately 28.4 kDa, and is fully biologically active following purification.
Molwt:
28.4 kDa (Reducing conditions)
Purity:
>95%

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